During the pregnancy, there happens the changes in the constituents of proteins in the maternal blood. Such proteins were classified as pregnancy proteins (Hong and Shim, 1978; Wurz, 1979; Park, 1982 b; Bohn and Dati, 1983).
The pregnancy proteins can be divided into two categories: the pregnancy-specific proteins and the pregnancy-associated proteins. The pregnancy-specific proteins do not occur in normal sera; they all appear to be synthesized in the trophoblast whence they are secreted into the maternal blood stream during gravidity. The pregnancy-associated proteins on the other hand do not originate in the trophoblast; they are already present in trace amounts in all normal sera. Plasma concentrations of those proteins are also often raised in the conditions other than pregnancy (Wiirz, 1979; Park, 1982 b; Bohn and Dati, 1983).
Most of the´ pregnancy proteins were found to be valuable in some way or another in pregnancy and/ or tumor diagnosis. Their detection or determination may serve either as pregnancy test (Gordon et al., 1977 Wiirz` et al., 1981; Park, 1982 b; Kwon et al., 1983), as indicator of placental function (Pluta et al., 1977; Wurz, 1979; Park, 1982 b), or as tumor marker for monitoring patient with malignant diseases (Johnson et al., 1977; Searle et al., 1978; Rosen et al., 1979; Lange et al., 1980 Park et al., 1984). Recently someimmunosuppressive actions were also suggested (Tomoda et al., 1976; Park, 1982 a; Park et al., 1983).
In addition to the pregnancy-specific proteins, a number of other placental proteins have been detected and characterized recently; they are mainly constituents of the placental tissue and not so much secreted into the maternal blood stream (Bohn and Dati, 1983).
Park and his associators have tried to determine the serum amounts of pregnancy protein in pregnant - women (Kwon et al., 1983), in infants (Kim et al., 1983) and in patients with various malignancies (Park et al., 1984), to localize the sites of synthesis and storage (Park and Kim, 1980; Kim and Park, 1982) and to confirm their effects on the immunologic action (Park, 1982 a; Park et al., 1983). However, in those studies, there were muclh difficulties because most of the pregnancy proteins were not available.
In order to make further study, the authors are asked to isolate each component of pregnancy proteins and identify them. Thus the author made this study according to Lin et al. (1974) method, using Sepharose 6B column chromatography, and confirmed their purities by high performance liquid chromatography, double diffusion method (Ouchterlony, 1949), Enzygnost-SP,, Gonavislide and Direkter Schwan-
gershaft test. T he auther could confirm the presence of pregnancy proteins within the eluates of Sepharose 6B column chromatography- using pregnant women´s sera, but failed to isolate them separately.
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